Models to describe the thermal development rates of Cycloneda sanguinea L. sanguinea correspond to distinct ecotypes and allopatric speciation occurred in different climatic regions followed by dispersal.
These findings support the idea that various ribotypes of A. Ribotype A grew better at lower temperatures compared to ribotype B which preferred higher temperatures. This differentiation supports the temperature optimum conditions that were established for these two ribotypes in the laboratory. sanguinea ribotype B bloomed only in the summer, corresponding to the summer type. sanguinea was present year-round in Xiamen Harbour, but it only bloomed in the winter and spring, thus corresponding to the winter type. sanguinea globally, with the ribotypes A and B co-occuring in Xiamen Harbour. These experiments showed four distinct ribotypes of A. To examine potential ecological differentiation of the distinct genotypes, growth responses of the studied strains were tested under laboratory conditions at temperatures of 12Â☌ to 33Â☌. For comparison with Chinese material, additional large subunit ribosomal DNA sequences were obtained for three established strains from Malaysian and French waters. These cells were sequenced by single-cell PCR. To investigate whether the observed cryptic diversity was related to environmental differentiation, 634 cells were obtained from seasonal water samples collected from 2008 to 2012. New details of the apical structure complex of Akashiwo sanguinea were also found. The cultures displayed identical morphology but were genetically different, thus revealing presence of cryptic diversity in the study area. Four clonal cultures were established by isolating single cells from Xiamen Harbour (the East China Sea) for morphological and genetic analyses. This cosmopolitan species includes several genetically differentiated clades.
Luo, Zhaohe Yang, Weidong Leaw, Chui Pin Pospelova, Vera Bilien, Gwenael Liow, Guat Ru Lim, Po Teen Gu, Haifengīlooms of the harmful dinoflagellate Akashiwo sanguinea are responsible for the mass mortality of fish and invertebrates in coastal waters. Journal compilation © 2009 Blackwell Publishing Ltd.Ĭryptic diversity within the harmful dinoflagellate Akashiwo sanguinea in coastal Chinese waters is related to differentiated ecological niches. These loci have the potential to reveal genetic structure and estimate gene flow among A. These loci were highly variable, with between 2 and 10 alleles per locus, and estimated gene diversity ranging from 0.08 to 0.82. We isolated 13 polymorphic microsatellites from the red-tide causing dinoflagellate Akashiwo sanguinea. sanguinea extract has both anti-mutagenic and anti-tumor effects.ĭevelopment of compound microsatellite markers in red-tide-causing dinoflagellate Akashiwo sanguinea (Dinophyceae).Ĭho, S-Y Nagai, S Nishitani, G Han, M-S The results of this study illustrate that C. Cell culture in vitro experiments show that there is no inhibition of growth or increase in cell death on normal mouse fibroblasts, but a stronger inhibition of cell growth and an increase in cell death of Hep-2 and MGC-803 tumor cells. sanguinea has anti-mutagenic effect, but no mutagenicity. We used Ames tests, mouse bone marrow polychromatic erythrocytes micronucleus tests, and tumor cells cultured in vitro to study the anti-mutagenic and anti-tumor effects of C. In order to explore their anti-cancer properties, edible wild plants from Xinjiang have been tested for their antitumor properties. Sun, Jianling Gao, Guolan Gao, YuLian Xiong, LiJuan Li, Xiaoying Guo, Jihong Zhang, YuemingĬrataegus sanguinea is a wild plant, which has been widely grown in the north and south of the Tianshan mountains in Xinjiang. Experimental research on the in vitro antitumor effects of Crataegus sanguinea.
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